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raw 264 7 cell monoculture  (ATCC)


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    Structured Review

    ATCC raw 264 7 cell monoculture
    In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).
    Raw 264 7 Cell Monoculture, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raw+264+7+cell+monoculture/pmc12813247-365-23-50?v=ATCC
    Average 99 stars, based on 24062 article reviews
    raw 264 7 cell monoculture - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair"

    Article Title: 4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.102691

    In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).
    Figure Legend Snippet: In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

    Techniques Used: In Vitro, Co-Culture Assay, Flow Cytometry, Quantitative RT-PCR, Immunofluorescence, Fluorescence

    3 cell culture models. Raw 264.7 cell monoculture model, rBMSCS cell monoculture model, and Raw264.7-rBMSCs cell co-culture model.
    Figure Legend Snippet: 3 cell culture models. Raw 264.7 cell monoculture model, rBMSCS cell monoculture model, and Raw264.7-rBMSCs cell co-culture model.

    Techniques Used: Cell Culture, Co-Culture Assay



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    raw 264 7 cell monoculture  (ATCC)
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    ATCC raw 264 7 cell monoculture
    In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).
    Raw 264 7 Cell Monoculture, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raw+264+7+cell+monoculture/pmc12813247-365-23-50?v=ATCC
    Average 99 stars, based on 1 article reviews
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    In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).
    Cell Monocultures, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/raw+264+7+cell+monoculture/pm37367618-33-0-6?v=ATCC
    Average 99 stars, based on 1 article reviews
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    99/100 stars
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    Image Search Results


    In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

    Journal: Materials Today Bio

    Article Title: 4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair

    doi: 10.1016/j.mtbio.2025.102691

    Figure Lengend Snippet: In vitro immunomodulatory properties of PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, NG-CSMA/Fe 3 O 4 -PLLA scaffolds. a-b) 3 days of co-culture of RAW 264.7 with PLLA, Fe 3 O 4 -PLLA, CSMA/Fe 3 O 4 -PLLA, and NG-CSMA/Fe 3 O 4 -PLLA scaffolds, flow cytometry was performed to analyze M1, M2 markers CD86 and CD206. c) mRNA levels of TNF-α, IL-1β, Arg-1, and CD206 by qRT-PCR; d) Immunofluorescence images of M1 (iNOS) and M2 (Arg-1) macrophage markers; and e) quantitative analysis of fluorescence intensity. n = 3. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001).

    Article Snippet: To assess the immunomodulatory properties and osteogenic capacity of each group of scaffolds evaluated in vitro , three different culture models were established: Raw 264.7 cell monoculture, rBMSCS cell monoculture and Raw 264.7-rBMSCs cell co-culture ( ). rBMSCs (RRID: CVCL_A9JU) and Raw 264.7 cells (RRID: CVCL_0493) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were authenticated according to the supplier's instructions to ensure the absence of contaminants.

    Techniques: In Vitro, Co-Culture Assay, Flow Cytometry, Quantitative RT-PCR, Immunofluorescence, Fluorescence

    3 cell culture models. Raw 264.7 cell monoculture model, rBMSCS cell monoculture model, and Raw264.7-rBMSCs cell co-culture model.

    Journal: Materials Today Bio

    Article Title: 4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair

    doi: 10.1016/j.mtbio.2025.102691

    Figure Lengend Snippet: 3 cell culture models. Raw 264.7 cell monoculture model, rBMSCS cell monoculture model, and Raw264.7-rBMSCs cell co-culture model.

    Article Snippet: To assess the immunomodulatory properties and osteogenic capacity of each group of scaffolds evaluated in vitro , three different culture models were established: Raw 264.7 cell monoculture, rBMSCS cell monoculture and Raw 264.7-rBMSCs cell co-culture ( ). rBMSCs (RRID: CVCL_A9JU) and Raw 264.7 cells (RRID: CVCL_0493) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and were authenticated according to the supplier's instructions to ensure the absence of contaminants.

    Techniques: Cell Culture, Co-Culture Assay